RedSafe™ – the non-toxic alternative to ethidium bromide – 3 for 2
*This promotion is valid until April 30, 2018. The offer applies to the list price and cannot be combined with other promotions, quotes or special prices.
Non-toxic substitute for ethidium bromide
Product Number: 21141
Availability: in stock
Delivery: 1 day
Size: 1 ml (20.000 x concentrated)
Price: 98,00 €
Use the promotion code RedSafe3for2 - get 3 tubes, pay only for 2*
RedSafe™ Nucleic Acid Staining Solution (20,000x) is a safe and economically priced nucleic acid staining solution. It's an alternative to the traditional ethidium bromide (EtBr) stain for detecting nucleic acids in agarose gels. It emits a green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 309 nm and another at 419 nm. In addition, it has one excitation maximum at 514 nm (visible range). The fluorescence emission of RedSafe™ bound to DNA is centered at 537 nm. RedSafe™ Nucleic Acid Staining Solution (20,000x) is as sensitive as EtBr. The protocol is also similar to using EtBr. The Ames test proves lower mutation with RedSafe™ Nucleic Acid Staining Solution compared to EtBr. In addition, RedSafe™ Nucleic Acid Staining Solution (20,000x) has a negative result in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test.
Use RedSafe™ Nucleic Acid Staining Solution to be on the safe side and improve the safety of your laboratory!
Q1: Is RedSafe suitable for UV illumination?
A1:Yes. RedSafe has two fluorescence excitation maxima when it is bound to nucleic acid. One is centered at 309 nm and another is at 419 nm. In addition, it has one excitation maximum at 514 nm (green).
Q2: Are the standard filters used for EtBr suitable for use with RedSafe?
A2: No. The red/orange filters for EB-stained gels should not be used with RedSafe stained gels. The filters for SYBR Green stained gels (at 494nm and 521nm) should be used. In addition, yellow, green gelation or cellophane filters can be used
Q3: Does RedSafe perform well with lower concentrations of DNA?
A3: Yes. However, smaller fragments of less than 300 bp are not as bright as the larger ones.
Q4: Can we use RedSafe for RNA as well as DNA?
Q5: We usually add our stain directly to the gel. Is it okay to cast RedSafe directly in the gel?
A5: Yes. RedSafe can be cast directly in the gel, loaded along with the DNA sample or used as a post-stain.
Q6: Should we add RedSafe to the running buffer?
A6: RedSafe can be added to the running buffer (25µl per ml). In general, this is only necessary if you are having issues with sections of the gel washing out (lower or higher intensity).
Q7: How can RedSafe be disposed of after use?
A7: RedSafe does not create any toxic waste. Therefore, it can be disposed according to laboratory regulations.
Q8: Can RedSafe be used as post-electrophoresis stain?
A8: Yes. Add 5 µl of RedSafe to 100 ml of buffer solution. You may need to adjust the concentration for optimal performance.
Q9: Will RedSafe work with low concentration of DNA ?
A9: RedSafe can work with low concentrations of DNA. However, smaller fragments of less than 300 bp may not be as bright.
Q10: Will RedSafe work with DNA using polyacrylamide gels?