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LiveLight™ cell culture media and supplements have been reformulated with specific phototoxic... more
LiveLight™ cell culture media and supplements have been reformulated with specific phototoxic components eliminated and replaced. LiveLight™ media and supplements allow prolonged exposure of cells to light whilst maintaining high levels of cell viability and functionality. LiveLight™ cell culture products do more than just reduce auto-fluorescence. They actively protect your cells from the lethal effects of photo-induced free radicals that are common components of standard media. Experiments with cultured cells, including fluorescence microscopy, optogenetics, fluorescence activated cell sorting (FACS), calcium imaging, super-resolution microscopy and automated cell culture, often entail high levels or prolonged exposure to light. However, cell culture media and supplements contain components that are converted to toxic free radicals by light. In particular, DMEM (Dulbecco’s Modified Eagle Medium) and Neurobasal® medium, as well as cell culture supplements such as B-27®, SATO and NS21, can lead to significant perturbation of cellular behavior and marked increases in cell death. This issue has been discussed, for example, in “Artifacts of Light”, Nature Methods (2013) volume 10 (12): 1135. LiveLight™ products generate very low levels of auto-fluorescence, dramatically improving the quality of data that can be obtained.
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