pluriSpin® Human CD45⁺ Cell Depletion, for the negative selection of cells, was developed to isolate a clean, vital and unaffected CD45⁺ free cell population from whole blood, buffy coat, umbilical cord blood, bone marrow or similar sample materials. The product labels the CD45⁺ cells and leaves the CD45 negative fraction untouched. By means of a subsequent density gradient centrifugation - PBMC Spin - the CD45⁺ cells are removed from the sample together with the erythrocytes (RBC) and granulocytes. The CD45 negative fraction remains enriched in the interphase between the plasma and the density medium.