The NEXTFLEX^® RiboNaut^™ rRNA depletion kit (human / mouse / rat) is an effective method to remove rRNA contamination while enabling labs to interrogate additional RNA species in a sample, not only limited to intact mRNAs. The kit utilizes subtractive hybridization technology consisting of a mixture of biotinylated oligos complementary to rRNAs to pull them out of the sample using streptavidin-coated magnetic beads. The kit has been optimized for use with 5 ng – 1 µg of total RNA as starting material to deplete cytoplasmic and mitochondrial rRNAs. The kit has been verified to be compatible with human, mouse and rat, and it may be compatible with other mammalian species.