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This specific ctDNA Mutation Mix v2 product has an allele frequency of 0.25%. Size distribution, library yield and complexity consistent with native ctDNA. Single sample with 40 clinically-relevant mutations across 28 genes, all at the same specified allele frequency. Offered as a purified DNA mixture for ease of use during assay development. Mutation targets precisely quantitated with digital PCR. Single well-characterized GM24385 human genomic DNA as background wild-type material. Manufactured in GMP-compliant and ISO 13485-certified facilities