Next-Generation Sequencing of Immunoglobulin Genes in Lymphoid Malignancies

The study of B-cell receptor rearrangements has impacted a myriad of fields in medicine, including oncology, immunology, and infectious disease. In cancer, lymphoid malignancies harbor clonal rearrangements that can aid in the initial recognition of lymphoma. Next-generation sequencing of the immunoglobulin genes has expanded our understanding of B-cell biology and has introduced new considerations for clonality assessment. In this session, Rena Xian of Johns Hopkins School of Medicine will review the genes involved, compare sequencing techniques, and illustrate special considerations related to immunoglobulin sequencing. 

Learning Objectives:

• Identify genes targeted for clonality assessment in B-cell malignancies
• Compare sequencing techniques used for immunoglobulin genes
• Describe pre-analytical variables that impact immunoglobulin sequencing
• Contrast clonality analysis by next-generation sequencing to traditional non-sequencing methods

Presenter

Rena Ruiyu Xian, M.D., M.S.
Assistant Professor of Pathology and Oncology | Johns Hopkins University School of Medicine | USA