The NEXTFLEX® Rapid DNA-Seq Kit 2.0 is designed for an approximately 3 hour DNA library construction with 1 ng – 1 μg of fragmented DNA. This kit is not recommended for FFPE samples. The kit can be used to prepare multiplexed libraries for single or paired-end sequencing using Illumina® platforms. In addition, the availability of up to 384 unique adapter barcodes facilitates high-throughput applications.

There are five main steps involved in preparing DNA for sequencing: DNA extraction, DNA fragmentation, DNA end repair / adenylation, adapter ligation, and PCR amplification. The NEXTFLEX® Rapid 2.0 DNA Seq Kit contains the necessary material to take the user’s purified and fragmented DNA through preparation and amplification for loading onto flow cells for sequencing.