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FLT3 ITD MRD Assay

FLT3 ITD MRD Assay

FLT3 ITD MRD Assay

#1-412-0019

This Research Use Only assay targets the juxtamembrane (JM) region of the FLT3 gene to identify ITD mutations using the Illumina MiSeq platform. For detection of internal tandem duplication (ITD) mutation in the FMS-like tyrosine kinase 3 (FLT3) gene.

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Delivery time 7 Workdays

Size: 96 rxns
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  • 1-412-0019
The FLT3 ITD MRD assay is designed to detect the presence of ITD mutations within exons 14/15 of... more

The FLT3 ITD MRD assay is designed to detect the presence of ITD mutations within exons 14/15 of the FLT3 gene in a given human DNA sample. The test is an amplicon-based approach which uses polymerase chain reaction (PCR) to amplify the region of interest and next-generation sequencing (NGS) to detect the region of interest. Primers included in the master mixes are designed with Illumina adapters containing unique indexes. This method allows for pooling of amplicons from several different samples onto one MiSeq™ flow cell, allowing for up to 24 samples to be analyzed in parallel in a single run. The assay includes twenty-four dual-indexed PCR master mixes, along with positive and negative controls. The FLT3 ITD MRD assay was designed with 24 unique index combinations, labeled as IA or IB. The associated RUO FLT3 ITD MRD v1.2 software provides a simple and streamlined method of analysis.

PROPERTIES
storage temperature: -70 °C
shipping method: dry ice
regulatory status: RUO
detection platform: Illumina
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