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Exo-spin standard, 8 columns, 60 ml buffer
Exo-spin™ products provide a proven, rapid and reliable way of generating high quality purified... more
Exo-spin™ products provide a proven, rapid and reliable way of generating high quality purified exosomes suitable for a range of research applications. Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), making it superior to other techniques that rely solely on one method. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. We provide a simple, two-step protocol which allows consistent and reliable purification of samples in under 2 hours. Each column in the EX01 Exo-spin™ kit enables processing of up to 50 ml of cell culture medium, saliva, urine, cerebrospinal fluid (CSF), human breast milk and other low-exosome-content biological fluids.
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