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Statistics & Tracking
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IdentiClone™ TCRB Gene Clonality Assay (ABI)
Three multiplex master mixes target conserved regions within the variable (Vβ), diversity (Dβ),... more
Three multiplex master mixes target conserved regions within the variable (Vβ), diversity (Dβ), and the joining (Jβ) regions that flank the unique hypervariable, antigen-binding, complementarity determining region 3 (CDR3) of the T-cell receptor beta locus. Tube A contains 23 Vβ primers, six Jβ1 primers, and three Jβ2 primers. Tube B contains 23 Vβ and four Jβ2 primers. Tube C contains two Dβ and 13 Jβ primers. Positive and negative DNA controls, as well as a Specimen Control Size Ladder Master Mix, are included. PCR products can be analyzed by capillary electrophoresis or heteroduplex analysis. Clonality is indicated, if any one of the master mixes generates clonal products.