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IGH Somatic Hypermutation Assay v2.0
2 master mixes for amplifying the regions Vƙ1-7and Jƙ1-5 which flank region 3 (CDR3). Further... more
2 master mixes for amplifying the regions Vƙ1-7and Jƙ1-5 which flank region 3 (CDR3). Further primers for the amplification of Kde and intragenic regions. Allows for bi-directional sequencing of clonal PCR products with just one sequencing-tag specific forward primer and one JH reverse primer, thus ensuring a more reliable and complete coverage of clonal products. Current ERIC (European Research Initiative on CLL) guidelines recommend bi-directional sequencing when determining the IGH SHM status.