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2 master mixes for amplifying the regions Vƙ1-7and Jƙ1-5 which flank region 3 (CDR3). Further primers for the amplification of Kde and intragenic regions. Allows for bi-directional sequencing of clonal PCR products with
just one sequencing-tag specific forward primer and one JH reverse primer, thus ensuring a more reliable and complete coverage of clonal products. Current ERIC (European Research Initiative on CLL) guidelines recommend bi-directional sequencing when determining the IGH SHM status.
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the usability of this website, serve for direct advertising or simplify interaction with other websites and social networks, will only be used with your consent.
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