The superior design of the NEXTFLEX® Unique Dual Index barcodes allows a broad range of multiplexing – users can multiplex anywhere between two samples and 1,536 samples in a single sequencing run. The wide range of multiplexing options enables barcode rotation (avoiding frequent use of the same barcode set) to mitigate run-to-run sample carryover and crosstalk. NEXTFLEX® Unique Dual Index barcodes are compatible with NEXTFLEX® library prep kits designed for Illumina® paired-end and single-read sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.