Single vial format for daily run use, 40 unique multiplexed variants across 28 genes, 20 SNVs, 5 SNVs that are part of homopolymers, 13 indels, 2 SVs. Mutation targets quantitated with digital PCR, well-characterized GM24385 human genomic DNA as background wild-type material. Manufactured within cGMP-compliant, ISO 13485-certified facilities. The Seraseq Tumor Mutation Mix-II DNA Mix v2 (AF10) HC is formulated for use with targeted next-generation sequencing (NGS) assays that detect mutations in key oncogenes and tumor suppressor genes. The Seraseq Tumor Mutation Mix-II DNA Mix v2 (AF10) HC is intended as a quality reference material for translational and disease research testing to monitor library preparation, sequencing, and variant allele detection under a given set of bioinformatics pipeline parameters.